SAE1 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
The SAE1 polyclonal antibody (Cat # PAB4352) is used in Western blot to detect SAE1 in mouse heart tissue lysate .
Western Blot (Transfected lysate)
Western blot analysis of SAE1 (arrow) using rabbit SAE1 polyclonal antibody (Cat # PAB4352). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SAE1 gene (Lane 2) (Origene Technologies).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with SAE1 polyclonal antibody (Cat # PAB4352) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of SAE1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human SAE1.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
The SAE1 polyclonal antibody (Cat # PAB4352) is used in Western blot to detect SAE1 in mouse heart tissue lysate .Western Blot (Transfected lysate)
Western blot analysis of SAE1 (arrow) using rabbit SAE1 polyclonal antibody (Cat # PAB4352). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SAE1 gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with SAE1 polyclonal antibody (Cat # PAB4352) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .Enzyme-linked Immunoabsorbent Assay
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Gene Info — SAE1
Entrez GeneID
10055Protein Accession#
NP_005491;Q9UBE0Gene Name
SAE1
Gene Alias
AOS1, FLJ3091, HSPC140, SUA1
Gene Description
SUMO1 activating enzyme subunit 1
Gene Ontology
HyperlinkOther Designations
SUMO-1 activating enzyme E1 N subunit|SUMO-1 activating enzyme subunit 1|activator of SUMO1|sentrin/SUMO-activating protein AOS1|ubiquitin-like protein SUMO-1 activating enzyme
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Interactome
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Pathway
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Publication Reference
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Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1.
Desterro JM, Rodriguez MS, Kemp GD, Hay RT.
The Journal of Biological Chemistry 1999 Apr; 274(15):10618.
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Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex.
Gong L, Li B, Millas S, Yeh ET.
FEBS Letters 1999 Apr; 448(1):185.
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In vitro SUMO-1 modification requires two enzymatic steps, E1 and E2.
Okuma T, Honda R, Ichikawa G, Tsumagari N, Yasuda H.
Biochemical and Biophysical Research Communications 1999 Jan; 254(3):693.
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Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1.
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