HSF1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of HSF1 polyclonal antibody (Cat # PAB4339) in 293 cell line lysates . Non-sumoylated HSF1 (arrow) was detected using the purified HSF1 polyclonal antibody (Cat # PAB4339) .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of HSF1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to human HSF1.
Sequence
PLVRVKEEPPSPPQSPR
Host
Rabbit
Reactivity
Human
Specificity
This antibody recognize synthetic peptide (PLVRVKEEPPSPPQSPR) containing a predicted sumoylation site of human HSF1.
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HSF1 polyclonal antibody (Cat # PAB4339) in 293 cell line lysates . Non-sumoylated HSF1 (arrow) was detected using the purified HSF1 polyclonal antibody (Cat # PAB4339) .Enzyme-linked Immunoabsorbent Assay
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Gene Info — HSF1
Entrez GeneID
3297Protein Accession#
NP_005517;Q00613Gene Name
HSF1
Gene Alias
HSTF1
Gene Description
heat shock transcription factor 1
Omim ID
140580Gene Ontology
HyperlinkGene Summary
The product of this gene is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of this gene. [provided by RefSeq
Other Designations
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Interactome
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Publication Reference
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Regulation of molecular chaperone gene transcription involves the serine phosphorylation, 14-3-3 epsilon binding, and cytoplasmic sequestration of heat shock factor 1.
Wang X, Grammatikakis N, Siganou A, Calderwood SK.
Molecular and Cellular Biology 2003 Sep; 23(17):6013.
Application：IF, IP, WB-Tr, Human, HeLa cells.
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Elevated expression of heat shock factor (HSF) 2A stimulates HSF1-induced transcription during stress.
He H, Soncin F, Grammatikakis N, Li Y, Siganou A, Gong J, Brown SA, Kingston RE, Calderwood SK.
The Journal of Biological Chemistry 2003 Sep; 278(38):35465.
Application：WB-Tr, Human, Mouse, K562, MEL cells.
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Insights into the regulation of heat shock transcription factor 1 SUMO-1 modification.
Hilgarth RS, Hong Y, Park-Sarge OK, Sarge KD.
Biochemical and Biophysical Research Communications 2003 Mar; 303(1):196.
Application：WB, Human, HeLa cells.
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Regulation of molecular chaperone gene transcription involves the serine phosphorylation, 14-3-3 epsilon binding, and cytoplasmic sequestration of heat shock factor 1.
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