PIGK polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
The PIGK polyclonal antibody (Cat # PAB4335) is used in Western blot to detect PIGK in mouse liver tissue lysate .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PIGK polyclonal antibody (Cat # PAB4335) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PIGK.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human PIGK.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
The PIGK polyclonal antibody (Cat # PAB4335) is used in Western blot to detect PIGK in mouse liver tissue lysate .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PIGK polyclonal antibody (Cat # PAB4335) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .Enzyme-linked Immunoabsorbent Assay
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Gene Info — PIGK
Entrez GeneID
10026Protein Accession#
GPI8_HUMANGene Name
PIGK
Gene Alias
GPI8, MGC22559
Gene Description
phosphatidylinositol glycan anchor biosynthesis, class K
Omim ID
605087Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the cysteine protease family C13 that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is a member of the multisubunit enzyme, GPI transamidase and is thought to be its enzymatic component. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins. [provided by RefSeq
Other Designations
GPI transamidase subunit|OTTHUMP00000011255|phosphatidylinositol glycan, class K
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Interactome
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Pathway
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Disease
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Publication Reference
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Two subunits of glycosylphosphatidylinositol transamidase, GPI8 and PIG-T, form a functionally important intermolecular disulfide bridge.
Ohishi K, Nagamune K, Maeda Y, Kinoshita T.
The Journal of Biological Chemistry 2003 Apr; 278(16):13959.
Application：WB, Human, HeLa, K562 cells .
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PIG-S and PIG-T, essential for GPI anchor attachment to proteins, form a complex with GAA1 and GPI8.
Ohishi K, Inoue N, Kinoshita T.
The EMBO Journal 2001 Aug; 20(15):4088.
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Two subunits of glycosylphosphatidylinositol transamidase, GPI8 and PIG-T, form a functionally important intermolecular disulfide bridge.
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