MGAT1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MGAT1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human MGAT1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of MGAT1 polyclonal antibody (Cat # PAB4305) in Y-79 cell line lysate . MGAT1 (arrow) was detected using the purified MGAT1 polyclonal antibody (Cat # PAB4305) .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with MGAT1 polyclonal antibody (Cat # PAB4305) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . HC = hepatocarcinoma .Enzyme-linked Immunoabsorbent Assay
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Gene Info — MGAT1
Entrez GeneID
4245Protein Accession#
GNT1_HUMANGene Name
MGAT1
Gene Alias
GLCNAC-TI, GLCT1, GLYT1, GNT-1, GNT-I, MGAT
Gene Description
mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase
Omim ID
160995Gene Ontology
HyperlinkGene Summary
There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I is a medial-Golgi enzyme essential for the synthesis of hybrid and complex N-glycans. The protein, encoded by a single exon, shows typical features of a type II transmembrane protein. The protein is believed to be essential for normal embryogenesis. Several variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase|OTTHUMP00000161546
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Interactome
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Pathway
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Disease
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Publication Reference
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The human UDP-N-acetylglucosamine: alpha-6-D-mannoside-beta-1,2- N-acetylglucosaminyltransferase II gene (MGAT2). Cloning of genomic DNA, localization to chromosome 14q21, expression in insect cells and purification of the recombinant protein.
Tan J, D'Agostaro AF, Bendiak B, Reck F, Sarkar M, Squire JA, Leong P, Schachter H.
European Journal of Biochemistry 1995 Jul; 231(2):317.
Application:WB-Ce, WB-Tr, Human, Mammalian cells.
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Cloning and expression of the murine gene and chromosomal location of the human gene encoding N-acetylglucosaminyltransferase I.
Kumar R, Yang J, Eddy RL, Byers MG, Shows TB, Stanley P.
Glycobiology 1992 Aug; 2(4):383.
Application:WB-Ce, WB-Tr, Human, Mammalian cells.
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Organization and localization to chromosome 5 of the human UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I gene.
Hull E, Sarkar M, Spruijt MP, Hoppener JW, Dunn R, Schachter H.
Biochemical and Biophysical Research Communications 1991 Apr; 176(2):608.
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The human UDP-N-acetylglucosamine: alpha-6-D-mannoside-beta-1,2- N-acetylglucosaminyltransferase II gene (MGAT2). Cloning of genomic DNA, localization to chromosome 14q21, expression in insect cells and purification of the recombinant protein.
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