ARL1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of K-562 cell lysate (35 ug/lane) with ARL1 polyclonal antibody (Cat # PAB4256).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with ARL1 polyclonal antibody (Cat # PAB4256) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . HC = hepatocarcinoma .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ARL1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human ARL1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of K-562 cell lysate (35 ug/lane) with ARL1 polyclonal antibody (Cat # PAB4256).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with ARL1 polyclonal antibody (Cat # PAB4256) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . HC = hepatocarcinoma .Enzyme-linked Immunoabsorbent Assay
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Gene Info — ARL1
Entrez GeneID
400Protein Accession#
NP_001168;P40616Gene Name
ARL1
Gene Alias
ARFL1
Gene Description
ADP-ribosylation factor-like 1
Omim ID
603425Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the ARL (ADP-ribosylation factor-like) family of proteins, which are structurally related to ADP-ribosylation factors (ARFs). ARFs, described as activators of cholera toxin (CT) ADP-ribosyltransferase activity, regulate intracellular vesicular membrane trafficking, and stimulate a phospholipase D (PLD) isoform. Although, ARL proteins were initially thought not to activate CT or PLD, later work showed that they are weak stimulators of PLD and CT in a phospholipid dependent manner. [provided by RefSeq
Other Designations
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Interactome
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Publication Reference
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Phospholipid- and GTP-dependent activation of cholera toxin and phospholipase D by human ADP-ribosylation factor-like protein 1 (HARL1).
Hong JX, Lee FJ, Patton WA, Lin CY, Moss J, Vaughan M.
The Journal of Biological Chemistry 1998 Jun; 273(25):15872.
Application：WB-Re, WB-Ti, Human, Brains, Hearts, Kidneys, Livers, Lungs, Recombinant proteins, Skeletal muscles.
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Different ARF domains are required for the activation of cholera toxin and phospholipase D.
Zhang GF, Patton WA, Lee FJ, Liyanage M, Han JS, Rhee SG, Moss J, Vaughan M.
The Journal of Biological Chemistry 1995 Jan; 270(1):21.
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Phospholipid- and GTP-dependent activation of cholera toxin and phospholipase D by human ADP-ribosylation factor-like protein 1 (HARL1).
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