USP14 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
The USP14 polyclonal antibody (Cat # PAB4199) is used in Western blot to detect USP14 in HeLa cell lysates . Transfection data is kindly provided by Dr . B . Pierrat from the Novartis Institute for Biomedical Research (Basel, Switzerland) .
Western Blot (Cell lysate)
The USP14 polyclonal antibody (Cat # PAB4199) is used in Western blot to detect USP14 in A-375 . Transfection data is kindly provided by Dr . B . Pierrat from the Novartis Institute for Biomedical Research (Basel, Switzerland) .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with USP14 polyclonal antibody (Cat # PAB4199) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of USP14.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human USP14.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
The USP14 polyclonal antibody (Cat # PAB4199) is used in Western blot to detect USP14 in HeLa cell lysates . Transfection data is kindly provided by Dr . B . Pierrat from the Novartis Institute for Biomedical Research (Basel, Switzerland) .Western Blot (Cell lysate)
The USP14 polyclonal antibody (Cat # PAB4199) is used in Western blot to detect USP14 in A-375 . Transfection data is kindly provided by Dr . B . Pierrat from the Novartis Institute for Biomedical Research (Basel, Switzerland) .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with USP14 polyclonal antibody (Cat # PAB4199) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .Enzyme-linked Immunoabsorbent Assay
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Gene Info — USP14
Entrez GeneID
9097Protein Accession#
UB14_HUMANGene Name
USP14
Gene Alias
TGT
Gene Description
ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
Omim ID
607274Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
deubiquitinating enzyme 14|tRNA-guanine transglycosylase, 60-kD subunit|ubiquitin carboxyl-terminal hydrolase 14|ubiquitin specific protease 14|ubiquitin specific protease 14 (tRNA-guanine transglycosylase)|ubiquitin thiolesterase 14|ubiquitin-specific pr
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Interactome
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Publication Reference
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Deubiquitination of CXCR4 by USP14 is critical for both CXCL12-induced CXCR4 degradation and chemotaxis but not ERK ativation.
Mines MA, Goodwin JS, Limbird LE, Cui FF, Fan GH.
The Journal of Biological Chemistry 2008 Dec; 284(9):5742.
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Deubiquitination of CXCR4 by USP14 is critical for both CXCL12-induced CXCR4 degradation and chemotaxis but not ERK ativation.
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