DUSP8 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of DUSP8 polyclonal antibody (Cat # PAB4135) in HeLa cell lysate (35 ug/lane). DUSP8 (arrow) was detected using the purified polyclonal antibody (1 : 50 dilution).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with DUSP8 polyclonal antibody (Cat # PAB4135) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of DUSP8.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human DUSP8.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of DUSP8 polyclonal antibody (Cat # PAB4135) in HeLa cell lysate (35 ug/lane). DUSP8 (arrow) was detected using the purified polyclonal antibody (1 : 50 dilution).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with DUSP8 polyclonal antibody (Cat # PAB4135) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Enzyme-linked Immunoabsorbent Assay
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Gene Info — DUSP8
Entrez GeneID
1850Protein Accession#
NP_004411Gene Name
DUSP8
Gene Alias
C11orf81, FLJ42476, FLJ42958, HB5, HVH-5, HVH8
Gene Description
dual specificity phosphatase 8
Omim ID
602038Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates SAPK/JNK and p38, is expressed predominantly in the adult brain, heart, and skeletal muscle, is localized in the cytoplasm, and is induced by nerve growth factor and insulin. An intronless pseudogene for DUSP8 is present on chromosome 10q11.2. [provided by RefSeq
Other Designations
H1 phosphatase, vaccinia virus homolog|serine/threonine specific protein phosphatase
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Interactome
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Pathway
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Publication Reference
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Identification of a transcriptionally active hVH-5 pseudogene on 10q22.2.
Berger IR, Buschbeck M, Bange J, Ullrich A.
Cancer Genetics and Cytogenetics 2005 Jun; 159(2):155.
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Genomic organization and chromosomal localization of a member of the MAP kinase phosphatase gene family to human chromosome 11p15.5 and a pseudogene to 10q11.2.
Nesbit MA, Hodges MD, Campbell L, de Meulemeester TM, Alders M, Rodrigues NR, Talbot K, Theodosiou AM, Mannens MA, Nakamura Y, Little PF, Davies KE.
Genomics 1997 Jun; 42(2):284.
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hVH-5: a protein tyrosine phosphatase abundant in brain that inactivates mitogen-activated protein kinase.
Martell KJ, Seasholtz AF, Kwak SP, Clemens KK, Dixon JE.
Journal of Neurochemistry 1995 Oct; 65(4):1823.
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Identification of a transcriptionally active hVH-5 pseudogene on 10q22.2.
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