DUSP4 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of DUSP4 polyclonal antibody (Cat # PAB4129) in mouse brain tissue lysate (35 ug/lane). DUSP4 (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with DUSP4 polyclonal antibody (Cat # PAB4129) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of DUSP4.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human DUSP4.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of DUSP4 polyclonal antibody (Cat # PAB4129) in mouse brain tissue lysate (35 ug/lane). DUSP4 (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with DUSP4 polyclonal antibody (Cat # PAB4129) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Enzyme-linked Immunoabsorbent Assay
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Gene Info — DUSP4
Entrez GeneID
1846Protein Accession#
NP_001385;Q13115Gene Name
DUSP4
Gene Alias
HVH2, MKP-2, MKP2, TYP
Gene Description
dual specificity phosphatase 4
Omim ID
602747Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK1, ERK2 and JNK, is expressed in a variety of tissues, and is localized in the nucleus. Two alternatively spliced transcript variants, encoding distinct isoforms, have been observed for this gene. In addition, multiple polyadenylation sites have been reported. [provided by RefSeq
Other Designations
MAP kinase phosphatase 2|VH1 homologous phosphatase 2|serine/threonine specific protein phosphatase
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Interactome
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Pathway
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Publication Reference
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Discordance between the binding affinity of mitogen-activated protein kinase subfamily members for MAP kinase phosphatase-2 and their ability to activate the phosphatase catalytically.
Chen P, Hutter D, Yang X, Gorospe M, Davis RJ, Liu Y.
The Journal of Biological Chemistry 2001 Aug; 276(31):29440.
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Chromosomal localization of three human dual specificity phosphatase genes (DUSP4, DUSP6, and DUSP7).
Smith A, Price C, Cullen M, Muda M, King A, Ozanne B, Arkinstall S, Ashworth A.
Genomics 1997 Jun; 42(3):524.
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The mitogen-activated protein kinase phosphatases PAC1, MKP-1, and MKP-2 have unique substrate specificities and reduced activity in vivo toward the ERK2 sevenmaker mutation.
Chu Y, Solski PA, Khosravi-Far R, Der CJ, Kelly K.
The Journal of Biological Chemistry 1996 Mar; 271(11):6497.
Application：IP, Human, Monkey, COS-7 cells, Human T cells.
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Discordance between the binding affinity of mitogen-activated protein kinase subfamily members for MAP kinase phosphatase-2 and their ability to activate the phosphatase catalytically.
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