MLST8 polyclonal antibody
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More Files
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Western Blot (Transfected lysate)
Western blot analysis of MLST8 polyclonal antibody (Cat # PAB4075) (arrow). 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MLST8 gene (Lane 2) (Origene Technologies).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with MLST8 polyclonal antibody (Cat # PAB4075) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of GBL.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to internal region of human GBL.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:250-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of MLST8 polyclonal antibody (Cat # PAB4075) (arrow). 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MLST8 gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with MLST8 polyclonal antibody (Cat # PAB4075) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Enzyme-linked Immunoabsorbent Assay
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Gene Info — MLST8
Entrez GeneID
64223Protein Accession#
NP_071767;Q9BVC4Gene Name
MLST8
Gene Alias
GBL, LST8, POP3, WAT1, GbetaL
Gene Description
MTOR associated protein, LST8 homolog (S. cerevisiae)
Gene Ontology
HyperlinkGene Summary
OTTHUMP00000159046
Other Designations
OTTHUMP00000159046
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Interactome
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Pathway
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Publication Reference
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Complete sequencing and characterization of 21,243 full-length human cDNAs.
Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, Wakamatsu A, Hayashi K, Sato H, Nagai K, Kimura K, Makita H, Sekine M, Obayashi M, Nishi T, Shibahara T, Tanaka T, Ishii S, Yamamoto J, Saito K, Kawai Y, Isono Y, Nakamura Y, Nagahari K, Murakami K, Yasuda T, Iwayanagi T, Wagatsuma M, Shiratori A, Sudo H, Hosoiri T, Kaku Y, Kodaira H, Kondo H, Sugawara M, Takahashi M, Kanda K, Yokoi T, Furuya T, Kikkawa E, Omura Y, Abe K, Kamihara K, Katsuta N, Sato K, Tanikawa M, Yamazaki M, Ninomiya K
Nature Genetics 2003 Dec; 36(1):40.
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Complete sequencing and characterization of 21,243 full-length human cDNAs.
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