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Last updated: 2014/12/14
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PIK3CG polyclonal antibody

  • Catalog # : PAB4069
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of PIK3CG.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human PIK3CG.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Recommend Usage:
  • ELISA (1:1000)
    Western Blot (1:50-100)
    Immunohistochemistry (1:10-50)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of PIK3CG polyclonal antibody (Cat # PAB4069) in HeLa cell lysate (35 ug/lane). PIK3CG (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis of PIK3CG polyclonal antibody (Cat # PAB4069) in 293 cell lysate transiently transfected with the PIK3CG gene (2 ug/lane). PIK3CG (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human muscle tissue reacted with PIK3CG polyclonal antibody (Cat # PAB4069) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
    This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 5294
  • Protein Accession#:
  • NP_002640;P48736
  • Gene Name:
  • PIK3CG
  • Gene Alias:
  • PI3CG,PI3K,PI3Kgamma,PIK3
  • Gene Description:
  • phosphoinositide-3-kinase, catalytic, gamma polypeptide
  • Gene Summary:
  • This gene encodes a protein that belongs to the pi3/pi4-kinase family of proteins. The gene product is an enzyme that phosphorylates phosphoinositides on the 3-hydroxyl group of the inositol ring. It is an important modulator of extracellular signals, including those elicited by E-cadherin-mediated cell-cell adhesion, which plays an important role in maintenance of the structural and functional integrity of epithelia. In addition to its role in promoting assembly of adherens junctions, the protein is thought to play a pivotal role in the regulation of cytotoxicity in NK cells. The gene is located in a commonly deleted segment of chromosome 7 previously identified in myeloid leukemias. [provided by RefSeq
  • Other Designations:
  • 1-phosphatidylinositol 3-kinase,PI3-kinase,PTDINS-3-kinase,p110-gamma,phosphatidylinositol 3 kinase gamma, p110 gamma,phosphatidylinositol 3-kinase catalytic 110-kD gamma,phosphatidylinositol 3-kinase, catalytic, gamma polypeptide,phosphoinositide-3-kinas
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