GUCY1A2 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of GUCY1A2 polyclonal antibody (Cat # PAB4064) in NCI-H460 cell lysate (35 ug/lane). GUCY1A2 (arrow) was detected using the purified polyclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with GUCY1A2 polyclonal antibody (Cat # PAB4064) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of GUCY1A2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human GUCY1A2.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:50-200)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of GUCY1A2 polyclonal antibody (Cat # PAB4064) in NCI-H460 cell lysate (35 ug/lane). GUCY1A2 (arrow) was detected using the purified polyclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with GUCY1A2 polyclonal antibody (Cat # PAB4064) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Enzyme-linked Immunoabsorbent Assay
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Gene Info — GUCY1A2
Entrez GeneID
2977Protein Accession#
NP_000846Gene Name
GUCY1A2
Gene Alias
GC-SA2, GUC1A2
Gene Description
guanylate cyclase 1, soluble, alpha 2
Omim ID
601244Gene Ontology
HyperlinkGene Summary
Soluble guanylyl (or guanylate) cyclases are heterodimeric enzymes consisting of an alpha subunit, such as alpha-2 (GUCY1A2), and a beta subunit, typically beta-1 (GUCY1B3; MIM 139397), which are activated by nitric oxide (NO) and which catalyze conversion of GTP to 3-prime, 5-prime-cyclic GMP and pyrophosphate (Harteneck et al., 1991 [PubMed 1683630]).[supplied by OMIM
Other Designations
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Interactome
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Pathway
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Disease
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Publication Reference
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Expression of nNOS and soluble guanylate cyclase in schizophrenic brain.
Baba H, Suzuki T, Arai H, Emson PC.
Neuroreport 2004 Mar; 15(4):677.
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On the activation of soluble guanylyl cyclase by nitric oxide.
Bellamy TC, Wood J, Garthwaite J.
PNAS 2002 Jan; 99(1):507.
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Guanylyl cyclase/PSD-95 interaction: targeting of the nitric oxide-sensitive alpha2beta1 guanylyl cyclase to synaptic membranes.
Russwurm M, Wittau N, Koesling D.
The Journal of Biological Chemistry 2001 Nov; 276(48):44647.
Application：WB-Ti, Rat, Rat brains, Synaptosomes.
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Expression of nNOS and soluble guanylate cyclase in schizophrenic brain.
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