AK1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
The AK1 polyclonal antibody (Cat # PAB4040) is used in Western blot to detect AK1 in CHO cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with AK1 polyclonal antibody (Cat # PAB4040) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of AK1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human AK1.
Host
Rabbit
Reactivity
Hamster, Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
The AK1 polyclonal antibody (Cat # PAB4040) is used in Western blot to detect AK1 in CHO cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with AK1 polyclonal antibody (Cat # PAB4040) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Enzyme-linked Immunoabsorbent Assay
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Gene Info — AK1
Entrez GeneID
203Protein Accession#
NP_000467;P00568Gene Name
AK1
Gene Alias
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Gene Description
adenylate kinase 1
Omim ID
103000Gene Ontology
HyperlinkGene Summary
Adenylate kinase is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate group among adinine nucleotides. Three isozymes of adenylate kinase have been identified in vertebrates, adenylate isozyme 1 (AK1), 2 (AK2) and 3 (AK3). AK1 is found in the cytosol of skeletal muscle, brain and erythrocytes, whereas AK2 and AK3 are found in the mitochondria of other tissues including liver and heart. AK1 was identified because of its association with a rare genetic disorder causing nonspherocytic hemolytic anemia where a mutation in the AK1 gene was found to reduce the catalytic activity of the enzyme. [provided by RefSeq
Other Designations
ATP-AMP transphosphorylase|OTTHUMP00000022217|OTTHUMP00000022218|myokinase
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Interactome
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Pathway
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Disease
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Publication Reference
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Red cell adenylate kinase deficiency: molecular study of 3 new mutations (118G>A, 190G>A, and GAC deletion) associated with hereditary nonspherocytic hemolytic anemia.
Corrons JL, Garcia E, Tusell JJ, Varughese KI, West C, Beutler E.
Blood 2003 Jul; 102(1):353.
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Congenital haemolytic anaemia associated with adenylate kinase deficiency.
Toren A, Brok-Simoni F, Ben-Bassat I, Holtzman F, Mandel M, Neumann Y, Ramot B, Rechavi G, Kende G.
British Journal of Haematology 1994 Jun; 87(2):376.
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Regional assignment of the loci for adenylate kinase to 9q32 and for alpha 1-acid glycoprotein to 9q31-q32. A locus for Goltz syndrome in region 9q32-qter?
Zuffardi O, Caiulo A, Maraschio P, Tupler R, Bianchi E, Amisano P, Beluffi G, Moratti R, Liguri G.
Human Genetics 1989 Apr; 82(1):17.
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Red cell adenylate kinase deficiency: molecular study of 3 new mutations (118G>A, 190G>A, and GAC deletion) associated with hereditary nonspherocytic hemolytic anemia.
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