YWHAB polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
The YWHAB polyclonal antibody (Cat # PAB4033) is used in Western blot to detect YWHAB in Jurkat cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with YWHAB polyclonal antibody (Cat # PAB4033) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of YWHAB.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human YWHAB.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
The YWHAB polyclonal antibody (Cat # PAB4033) is used in Western blot to detect YWHAB in Jurkat cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with YWHAB polyclonal antibody (Cat # PAB4033) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Enzyme-linked Immunoabsorbent Assay
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Gene Info — YWHAB
Entrez GeneID
7529Protein Accession#
P31946Gene Name
YWHAB
Gene Alias
GW128, HS1, KCIP-1
Gene Description
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide
Omim ID
601289Gene Ontology
HyperlinkGene Summary
This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq
Other Designations
14-3-3 protein beta/alpha|OTTHUMP00000031075|OTTHUMP00000031076|brain protein 14-3-3, beta isoform|protein 1054|protein kinase C inhibitor protein-1
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Interactome
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Pathway
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Disease
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Publication Reference
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Immunoexpression of 14-3-3 proteins in glial cytoplasmic inclusions of multiple system atrophy.
Komori T, Ishizawa K, Arai N, Hirose T, Mizutani T, Oda M.
Acta Neuropathologica 2003 Jul; 106(1):66.
Application：IHC-P, Human, Brains from patients with multiple system atrophy.
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14-3-3beta is a p90 ribosomal S6 kinase (RSK) isoform 1-binding protein that negatively regulates RSK kinase activity.
Cavet ME, Lehoux S, Berk BC.
The Journal of Biological Chemistry 2003 Mar; 278(20):18376.
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The p38 and MK2 kinase cascade phosphorylates tuberin, the tuberous sclerosis 2 gene product, and enhances its interaction with 14-3-3.
Li Y, Inoki K, Vacratsis P, Guan KL.
The Journal of Biological Chemistry 2003 Feb; 278(16):13663.
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Immunoexpression of 14-3-3 proteins in glial cytoplasmic inclusions of multiple system atrophy.
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