NME4 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
The NME4 polyclonal antibody (Cat # PAB4030) is used in Western blot to detect NME4 in Jurkat cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with NME4 polyclonal antibody (Cat # PAB4030) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of NME4.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human NME4.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
The NME4 polyclonal antibody (Cat # PAB4030) is used in Western blot to detect NME4 in Jurkat cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with NME4 polyclonal antibody (Cat # PAB4030) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Enzyme-linked Immunoabsorbent Assay
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Gene Info — NME4
Entrez GeneID
4833Protein Accession#
O00746Gene Name
NME4
Gene Alias
NDPK-D, NM23H4, nm23-H4
Gene Description
non-metastatic cells 4, protein expressed in
Omim ID
601818Gene Ontology
HyperlinkGene Summary
The nucleoside diphosphate (NDP) kinases (EC 2.7.4.6) are ubiquitous enzymes that catalyze transfer of gamma-phosphates, via a phosphohistidine intermediate, between nucleoside and dioxynucleoside tri- and diphosphates. The enzymes are products of the nm23 gene family, which includes NME4 (Milon et al., 1997 [PubMed 9099850]).[supplied by OMIM
Other Designations
NDP kinase D|nucleoside-diphosphate kinase 4
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Interactome
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Pathway
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Publication Reference
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nm23-H4, a new member of the family of human nm23/nucleoside diphosphate kinase genes localised on chromosome 16p13.
Milon L, Rousseau-Merck MF, Munier A, Erent M, Lascu I, Capeau J, Lacombe ML.
Human Genetics 1997 Apr; 99(4):550.
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nm23-H4, a new member of the family of human nm23/nucleoside diphosphate kinase genes localised on chromosome 16p13.
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