PFKFB3 polyclonal antibody
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More Files
- More Functions
Western Blot
The PFKFB3 polyclonal antibody (Cat # PAB4017) is used in Western blot to detect PFKFB3 in Jurkat cell lysate (Lane 1) and mouse brain tissue lysate (Lane 2) .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with PFKFB3 polyclonal antibody (Cat # PAB4017) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PFKFB3.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human PFKFB3.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
The PFKFB3 polyclonal antibody (Cat # PAB4017) is used in Western blot to detect PFKFB3 in Jurkat cell lysate (Lane 1) and mouse brain tissue lysate (Lane 2) .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with PFKFB3 polyclonal antibody (Cat # PAB4017) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PFKFB3
Entrez GeneID
5209Protein Accession#
Q16875Gene Name
PFKFB3
Gene Alias
IPFK2, PFK2
Gene Description
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3
Omim ID
605319Gene Ontology
HyperlinkGene Summary
O
Other Designations
6-phosphofructo-2-kinase/ fructose-2,6-bisphosphatase|6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase|OTTHUMP00000019040|OTTHUMP00000044861|fructose-6-phosphate,2-kinase/fructose-2, 6-bisphosphatase|inducible 6-phosphofructo-2-kinase/fructose-2,6-bi
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Interactome
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Pathway
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Disease
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Publication Reference
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The human ubiquitous 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene (PFKFB3): promoter characterization and genomic structure.
Navarro-Sabate A, Manzano A, Riera L, Rosa JL, Ventura F, Bartrons R.
Gene 2001 Feb; 264(1):131.
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An inducible gene product for 6-phosphofructo-2-kinase with an AU-rich instability element: role in tumor cell glycolysis and the Warburg effect.
Chesney J, Mitchell R, Benigni F, Bacher M, Spiegel L, Al-Abed Y, Han JH, Metz C, Bucala R.
PNAS 1999 Mar; 96(6):3047.
Application：WB-Ce, WB-Tr, Human, HL-60, Human peripheral blood leukocytes, K-562, KG1a, MOLT4 cells.
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Characterization of a human placental fructose-6-phosphate, 2-kinase/fructose-2,6-bisphosphatase.
Sakakibara R, Kato M, Okamura N, Nakagawa T, Komada Y, Tominaga N, Shimojo M, Fukasawa M.
Journal of Biochemistry 1997 Jul; 122(1):122.
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The human ubiquitous 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene (PFKFB3): promoter characterization and genomic structure.
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