PFKP polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PFKP.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human PFKP.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
The PFKP polyclonal antibody (Cat # PAB3996) is used in Western blot to detect PFKP in Ramos cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with PFKP polyclonal antibody (Cat # PAB3996) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PFKP
Entrez GeneID
5214Protein Accession#
Q01813Gene Name
PFKP
Gene Alias
FLJ40226, PFK-C, PFKF
Gene Description
phosphofructokinase, platelet
Omim ID
171840Gene Ontology
HyperlinkGene Summary
The PFKP gene encodes the platelet isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The PFKP gene, which maps to chromosome 10p, is also expressed in fibroblasts. See also the muscle (PFKM; MIM 610681) and liver (PFKL; MIM 171860) isoforms of phosphofructokinase, which map to chromosomes 12q13 and 21q22, respectively. Vora (1981) [PubMed 6451249] determined that full tetrameric phophofructokinase enzyme expressed in platelets can be composed of subunits P4, P3L, and P2L2.[supplied by OMIM
Other Designations
OTTHUMP00000018966|Phosphofructokinase, platelet type
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Interactome
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Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Fructose and mannose metabolism
- Galactose metabolism
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Disease
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Publication Reference
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Trifunctional chemical probes for the consolidated detection and identification of enzyme activities from complex proteomes.
Adam GC, Sorensen EJ, Cravatt BF.
Molecular & Cellular Proteomics 2002 Oct; 1(10):828.
Application:WB-Ce, WB-Tr, Human, Monkey, MDA-MB-231, COS-7 cells.
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Normalization and subtraction: two approaches to facilitate gene discovery.
Bonaldo MF, Lennon G, Soares MB.
Genome Research 1996 Sep; 6(9):791.
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Regional chromosomal assignment of the human platelet phosphofructokinase gene to 10p15.
N Morrison, C Simpson, L Fothergill-Gilmore, E Boyd, J M Connor.
Human Genetics 1992 Apr; 89(1):105.
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Trifunctional chemical probes for the consolidated detection and identification of enzyme activities from complex proteomes.
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