EIF4EBP1 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of EIF4EBP1 (arrow) using rabbit EIF4EBP1 polyclonal antibody (Cat # PAB3954). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EIF4EBP1 gene (Lane 2) (Origene Technologies).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with EIF4EBP1 polyclonal antibody (Cat # PAB3954) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of EIF4EBP1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to residues surrounding T69 of human EIF4EBP1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:10-50)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of EIF4EBP1 (arrow) using rabbit EIF4EBP1 polyclonal antibody (Cat # PAB3954). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EIF4EBP1 gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with EIF4EBP1 polyclonal antibody (Cat # PAB3954) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. -
Gene Info — EIF4EBP1
Entrez GeneID
1978Protein Accession#
NP_004086Gene Name
EIF4EBP1
Gene Alias
4E-BP1, 4EBP1, BP-1, MGC4316, PHAS-I
Gene Description
eukaryotic translation initiation factor 4E binding protein 1
Omim ID
602223Gene Ontology
HyperlinkGene Summary
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq
Other Designations
eIF4E-binding protein 1|phosphorylated heat- and acid-stable protein regulated by insulin 1
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Interactome
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Pathway
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Disease
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Publication Reference
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4E-binding protein 1: a key molecular "funnel factor" in human cancer with clinical implications.
Armengol G, Rojo F, Castellvi J, Iglesias C, Cuatrecasas M, Pons B, Baselga J, Ramon y Cajal S.
Cancer Research 2007 Aug; 67(16):7551.
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PRAS40 is a target for mammalian target of rapamycin complex 1 and is required for signaling downstream of this complex.
Fonseca BD, Smith EM, Lee VH, MacKintosh C, Proud CG.
The Journal of Biological Chemistry 2007 Jun; 282(34):24514.
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4E-binding protein 1: a key molecular "funnel factor" in human cancer with clinical implications.
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