PIK3R1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PIK3R1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to residues surrounding Y580 of human PIK3R1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:10-50)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of PIK3R1 (arrow) using rabbit PIK3R1 polyclonal antibody (Cat # PAB3931). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIK3R1 gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PIK3R1 polyclonal antibody (Cat # PAB3931) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. -
Gene Info — PIK3R1
Entrez GeneID
5295Protein Accession#
NP_852556;P27986Gene Name
PIK3R1
Gene Alias
GRB1, p85, p85-ALPHA
Gene Description
phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
Omim ID
171833Gene Ontology
HyperlinkGene Summary
Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in three transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha)|phosphatidylinositol 3-kinase, regulatory, 1|phosphatidylinositol 3-kinase-associated p-85 alpha|phosphoinositide-3-kinase, regulatory subunit 1 (p85 alpha)|phosphoinositide-3-ki
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Interactome
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Pathway
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Disease
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Publication Reference
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Polarity and proliferation are controlled by distinct signaling pathways downstream of PI3-kinase in breast epithelial tumor cells.
Liu H, Radisky DC, Wang F, Bissell MJ.
Journal of Cellular Biology 2004 Feb; 164(4):603.
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Genetic down-regulation of phosphoinositide 3-kinase by bikunin correlates with suppression of invasion and metastasis in human ovarian cancer HRA cells.
Kobayashi H, Suzuki M, Kanayama N, Terao T.
The Journal of Biological Chemistry 2003 Nov; 279(8):6371.
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Activation of phosphatidylinositol 3-kinase/Akt pathway by androgen through interaction of p85alpha, androgen receptor, and Src.
Sun M, Yang L, Feldman RI, Sun XM, Bhalla KN, Jove R, Nicosia SV, Cheng JQ.
The Journal of Biological Chemistry 2003 Aug; 278(44):42992.
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Polarity and proliferation are controlled by distinct signaling pathways downstream of PI3-kinase in breast epithelial tumor cells.
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