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Last updated: 2014/7/7
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SOX2 polyclonal antibody

  • Catalog # : PAB3876
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of SOX2.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human SOX2.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Ammonium sulfate precipitation
  • Recommend Usage:
  • ELISA (1:1000)
    Western Blot (1:50-100)
    Immunohistochemistry (1:10-50)
    Immunofluorescence (1:10-50)
    Flow cytometry (1:10-50)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of SOX2 polyclonal antibody (Cat # PAB3876) in 293 cell line lysates (35 ug/lane). SOX2 (arrow) was detected using the purified polyclonal antibody.
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis of SOX2 (arrow) using rabbit SOX2 polyclonal antibody (Cat # PAB3876). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SOX2 gene (Lane 2) (Origene Technologies).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SOX2 polyclonal antibody (Cat # PAB3876) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of SOX2 polyclonal antibody (Cat # PAB3876) in HeLa cells. 0.025 mg/mL primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
  • ELISA
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of NCI-H292 cells using SOX2 polyclonal antibody (Cat # PAB3876)(bottom histogram) compared to a negative control cell (top histogram).
    FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 6657
  • Protein Accession#:
  • NP_003097;P48431
  • Gene Name:
  • SOX2
  • Gene Alias:
  • ANOP3,MCOPS3,MGC2413
  • Gene Description:
  • SRY (sex determining region Y)-box 2
  • Gene Summary:
  • This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq
  • Other Designations:
  • SRY-related HMG-box gene 2,sex-determining region Y-box 2,transcription factor SOX2
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