TDGF1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of TDGF1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human TDGF1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
The TDGF1 polyclonal antibody (Cat # PAB3871) is used in Western blot to detect TDGF1 in A-375 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with TDGF1 polyclonal antibody (Cat # PAB3871) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma -
Gene Info — TDGF1
Entrez GeneID
6997Protein Accession#
NP_003203;P13385Gene Name
TDGF1
Gene Alias
CR, CRGF, CRIPTO, Cripto-1
Gene Description
teratocarcinoma-derived growth factor 1
Omim ID
187395Gene Ontology
HyperlinkOther Designations
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Interactome
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Disease
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Publication Reference
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Nodal-dependent Cripto signaling promotes cardiomyogenesis and redirects the neural fate of embryonic stem cells.
Parisi S, D'Andrea D, Lago CT, Adamson ED, Persico MG, Minchiotti G.
The Journal of cell biology 2003 Oct; 163(2):303.
Application:WB-Tr, Human, HEK 293 cells.
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Cripto forms a complex with activin and type II activin receptors and can block activin signaling.
Gray PC, Harrison CA, Vale W.
PNAS 2003 Apr; 100(9):5193.
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Dual roles of Cripto as a ligand and coreceptor in the nodal signaling pathway.
Yan YT, Liu JJ, Luo Y, E C, Haltiwanger RS, Abate-Shen C, Shen MM.
Molecular and Cellular Biology 2002 Jul; 22(13):4439.
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Nodal-dependent Cripto signaling promotes cardiomyogenesis and redirects the neural fate of embryonic stem cells.
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