POU5F1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of POU5F1 polyclonal antibody (Cat # PAB3870) in HL-60 cell line lysates (35 ug/lane). POU5F1 (arrow) was detected using the purified polyclonal antibody (1:1000 dilution).
Immunofluorescence
Immunofluorescence analysis of POU5F1 polyclonal antibody (Cat # PAB3870) in HeLa cells. 0.025 mg/mL primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of POU5F1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to residues surrounding E125 of human POU5F1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
Immunofluorescence (1:10-50)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of POU5F1 polyclonal antibody (Cat # PAB3870) in HL-60 cell line lysates (35 ug/lane). POU5F1 (arrow) was detected using the purified polyclonal antibody (1:1000 dilution).Immunofluorescence
Immunofluorescence analysis of POU5F1 polyclonal antibody (Cat # PAB3870) in HeLa cells. 0.025 mg/mL primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI. -
Gene Info — POU5F1
Entrez GeneID
5460Protein Accession#
NP_002692;Q01860Gene Name
POU5F1
Gene Alias
MGC22487, OCT3, OCT4, OTF3, OTF4
Gene Description
POU class 5 homeobox 1
Omim ID
164177Gene Ontology
HyperlinkGene Summary
class 5
Other Designations
OTTHUMP00000029292|POU domain, class 5, transcription factor 1|POU-type homeodomain-containing DNA-binding protein|octamer-binding transcription factor-3
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Interactome
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Disease
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Publication Reference
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Crystal structure of a POU/HMG/DNA ternary complex suggests differential assembly of Oct4 and Sox2 on two enhancers.
Remenyi A, Lins K, Nissen LJ, Reinbold R, Scholer HR, Wilmanns M.
Genes & Development 2003 Aug; 17(16):2048.
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POU5F1 (OCT3/4) identifies cells with pluripotent potential in human germ cell tumors.
Looijenga LH, Stoop H, de Leeuw HP, de Gouveia Brazao CA, Gillis AJ, van Roozendaal KE, van Zoelen EJ, Weber RF, Wolffenbuttel KP, van Dekken H, Honecker F, Bokemeyer C, Perlman EJ, Schneider DT, Kononen J, Sauter G, Oosterhuis JW.
Cancer Research 2003 May; 63(9):2244.
Application：IHC-P, Human, Human germ cell tumors.
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Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct4.
Nichols J, Zevnik B, Anastassiadis K, Niwa H, Klewe-Nebenius D, Chambers I, Scholer H, Smith A.
Cell 1998 Oct; 95(3):379.
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Crystal structure of a POU/HMG/DNA ternary complex suggests differential assembly of Oct4 and Sox2 on two enhancers.
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