PDHX polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of PDHX polyclonal antibody (Cat # PAB3861) in NCI-H460 cell line lysates (35 ug/lane).PDHX (arrow) was detected using the purified polyclonal antibody (1:1000 dilution).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocarcinomareacted with PDHX polyclonal antibody (Cat # PAB3861), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PDHX.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to residues surrounding T11 of human PDHX.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
Immunofluorescence (1:100)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:10-50)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of PDHX polyclonal antibody (Cat # PAB3861) in NCI-H460 cell line lysates (35 ug/lane).PDHX (arrow) was detected using the purified polyclonal antibody (1:1000 dilution).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocarcinomareacted with PDHX polyclonal antibody (Cat # PAB3861), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Immunofluorescence
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Gene Info — PDHX
Entrez GeneID
8050Protein Accession#
NP_003468;O00330Gene Name
PDHX
Gene Alias
DLDBP, E3BP, OPDX, PDX1, proX
Gene Description
pyruvate dehydrogenase complex, component X
Gene Ontology
HyperlinkGene Summary
The PDHX gene encodes component X of the pyruvate dehydrogenase (PDH) complex. For a detailed description of the pyruvate dehydrogenase complex, see MIM 300502. The mammalian PDH complex differs from that in E. coli and from the other mammalian alpha-keto acid dehydrogenases by the presence of a 53-kD protein called protein X. Component X binds to the E3 (MIM 238331) component of the PDH complex (Robinson et al., 1990 [PubMed 2112155]; Aral et al., 1997 [PubMed 9399911]).[supplied by OMIM
Other Designations
E3-binding protein|pyruvate dehydrogenase complex, lipoyl-containing component X
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Interactome
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Publication Reference
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Detection of a homozygous four base pair deletion in the protein X gene in a case of pyruvate dehydrogenase complex deficiency.
Ling M, McEachern G, Seyda A, MacKay N, Scherer SW, Bratinova S, Beatty B, Giovannucci-Uzielli ML, Robinson BH.
Human Molecular Genetics 1998 Mar; 7(3):501.
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Mutations in PDX1, the human lipoyl-containing component X of the pyruvate dehydrogenase-complex gene on chromosome 11p1, in congenital lactic acidosis.
Aral B, Benelli C, Ait-Ghezala G, Amessou M, Fouque F, Maunoury C, Creau N, Kamoun P, Marsac C.
American Journal of Human Genetics 1997 Dec; 61(6):1318.
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Dihydrolipoamide dehydrogenase-binding protein of the human pyruvate dehydrogenase complex. DNA-derived amino acid sequence, expression, and reconstitution of the pyruvate dehydrogenase complex.
Harris RA, Bowker-Kinley MM, Wu P, Jeng J, Popov KM.
The Journal of Biological Chemistry 1997 Aug; 272(32):19746.
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Detection of a homozygous four base pair deletion in the protein X gene in a case of pyruvate dehydrogenase complex deficiency.
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