ZNF219 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of ZNF219 polyclonal antibody (Cat # PAB3822) in 293 cell line lysates (35 ug/lane).ZNF219 (arrow) was detected using the purified polyclonal antibody (1:1000 dilution).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human testisreacted with ZNF219 polyclonal antibody (Cat # PAB3822), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ZNF219.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human ZNF219.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:10-50)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of ZNF219 polyclonal antibody (Cat # PAB3822) in 293 cell line lysates (35 ug/lane).ZNF219 (arrow) was detected using the purified polyclonal antibody (1:1000 dilution).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human testisreacted with ZNF219 polyclonal antibody (Cat # PAB3822), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. -
Gene Info — ZNF219
Entrez GeneID
51222Protein Accession#
NP_001095142;Q9P2Y4Gene Name
ZNF219
Gene Alias
ZFP219
Gene Description
zinc finger protein 219
Omim ID
605036Gene Ontology
HyperlinkOther Designations
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Interactome
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Publication Reference
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Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.
Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M.
Cell 2006 Nov; 127(3):635.
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The negative regulator of Gli, Suppressor of fused (Sufu), interacts with SAP18, Galectin3 and other nuclear proteins.
Paces-Fessy M, Boucher D, Petit E, Paute-Briand S, Blanchet-Tournier MF.
The Biochemical Journal 2004 Mar; 378(Pt 2):353.
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Identification of the DNA binding specificity of the human ZNF219 protein and its function as a transcriptional repressor.
Sakai T, Hino K, Wada S, Maeda H.
DNA Research 2003 Aug; 10(4):155.
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Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.
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