EIF4B polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of EIF4B.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to internal region of human EIF4B.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of EIF4B polyclonal antibody (Cat # PAB3774) in HepG2 cell line lysates (35 ug/lane). EIF4B (arrow) was detected using the purified polyclonal antibody (1:1000 dilution).Western Blot (Transfected lysate)
Western blot analysis of EIF4B polyclonal antibody (Cat # PAB3774) in 293 cell line lysates transiently transfected with the EIF4B gene (2 ug/lane). EIF4B (arrow) was detected using the purified polyclonal antibody (1:1000 dilution).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EIF4B polyclonal antibody (Cat # PAB3774) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. -
Gene Info — EIF4B
Entrez GeneID
1975Protein Accession#
NP_001408;Q8WYK5Gene Name
EIF4B
Gene Alias
EIF-4B, PRO1843
Gene Description
eukaryotic translation initiation factor 4B
Omim ID
603928Gene Ontology
HyperlinkOther Designations
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Interactome
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Pathway
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Publication Reference
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Herpes simplex virus virion host shutoff protein is stimulated by translation initiation factors eIF4B and eIF4H.
Doepker RC, Hsu WL, Saffran HA, Smiley JR.
Journal of Virology 2004 May; 78(9):4684.
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Solution structure and RNA interactions of the RNA recognition motif from eukaryotic translation initiation factor 4B.
Fleming K, Ghuman J, Yuan X, Simpson P, Szendroi A, Matthews S, Curry S.
Biochemistry 2003 Aug; 42(30):8966.
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Cloning and expression of eukaryotic initiation factor 4B cDNA: sequence determination identifies a common RNA recognition motif.
Milburn SC, Hershey JW, Davies MV, Kelleher K, Kaufman RJ.
The EMBO Journal 1990 Sep; 9(9):2783.
Application:WB-Tr, Monkey, COS-1 cells.
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Herpes simplex virus virion host shutoff protein is stimulated by translation initiation factors eIF4B and eIF4H.
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