MRPL24 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MRPL24.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human MRPL24.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of MRPL24 polyclonal antibody (Cat # PAB3757) in mouse kidney tissue lysate (35 ug/lane). MRPL24 (arrow) was detected using the purified polyclonal antibody (1:1000 dilution). -
Gene Info — MRPL24
Entrez GeneID
79590Protein Accession#
NP_078816;Q96A35Gene Name
MRPL24
Gene Alias
FLJ20917, L24mt, MGC22737, MGC9831, MRP-L18, MRP-L24
Gene Description
mitochondrial ribosomal protein L24
Gene Ontology
HyperlinkGene Summary
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein which is more than twice the size of its E.coli counterpart (EcoL24). Sequence analysis identified two transcript variants that encode the same protein. [provided by RefSeq
Other Designations
39S ribosomal protein L24, mitochondrial|OTTHUMP00000038724|OTTHUMP00000038725
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Interactome
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Publication Reference
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Identification and characterization of over 100 mitochondrial ribosomal protein pseudogenes in the human genome.
Zhang Z, Gerstein M.
Genomics 2003 May; 81(5):468.
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The human mitochondrial ribosomal protein genes: mapping of 54 genes to the chromosomes and implications for human disorders.
Kenmochi N, Suzuki T, Uechi T, Magoori M, Kuniba M, Higa S, Watanabe K, Tanaka T.
Genomics 2001 Sep; 77(1-2):65.
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Mammalian mitochondrial ribosomal proteins (4). Amino acid sequencing, characterization, and identification of corresponding gene sequences.
O'Brien TW, Liu J, Sylvester JE, Mougey EB, Fischel-Ghodsian N, Thiede B, Wittmann-Liebold B, Graack HR.
The Journal of Biological Chemistry 2000 Jun; 275(24):18153.
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Identification and characterization of over 100 mitochondrial ribosomal protein pseudogenes in the human genome.
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