NUP50 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of NUP50 polyclonal antibody (Cat # PAB3709) in mouse brain tissue lysate. NUP50 (arrow) was detected using the purified polyclonal antibody.
Western Blot (Transfected lysate)
Western blot analysis of NUP50 (arrow) usingNUP50 polyclonal antibody (Cat # PAB3709). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NUP50 gene (Lane 2) (Origene Technologies).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of NUP50.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human NUP50.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of NUP50 polyclonal antibody (Cat # PAB3709) in mouse brain tissue lysate. NUP50 (arrow) was detected using the purified polyclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of NUP50 (arrow) usingNUP50 polyclonal antibody (Cat # PAB3709). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NUP50 gene (Lane 2) (Origene Technologies). -
Gene Info — NUP50
Entrez GeneID
10762Protein Accession#
NP_705931;Q8N6V5Gene Name
NUP50
Gene Alias
MGC39961, NPAP60, NPAP60L
Gene Description
nucleoporin 50kDa
Omim ID
604646Gene Ontology
HyperlinkGene Summary
The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000028685|nuclear pore-associated protein 60L
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Interactome
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Disease
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Publication Reference
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Nucleocytoplasmic transport: more than the usual suspects.
Swaminathan S, Melchior F.
Developmental Cell 2002 Sep; 3(3):304.
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Npap60/Nup50 is a tri-stable switch that stimulates importin-alpha:beta-mediated nuclear protein import.
Lindsay ME, Plafker K, Smith AE, Clurman BE, Macara IG.
Cell 2002 Aug; 110(3):349.
Application：IF, Human, Mouse, GSN2, HeLa, NIH3T3 cells.
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Characterization and targeted disruption of murine Nup50, a p27(Kip1)-interacting component of the nuclear pore complex.
Smitherman M, Lee K, Swanger J, Kapur R, Clurman BE.
Molecular and Cellular Biology 2000 Aug; 20(15):5631.
Application：IF, IP, WB-Ce, WB-Ti, WB-Tr, Human, Mouse, HeLa, NIH 3T3, 293 cells, MEFs, Embryo, Cerebrum, Cerebellum, Lungs, Heart, Kidney, Liver, Testes, Stomach.
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Nucleocytoplasmic transport: more than the usual suspects.
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