SIGLEC8 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of SIGLEC8.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human SIGLEC8.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
The SIGLEC8 polyclonal antibody (Cat # PAB3640) is used in Western blot to detect SIGLEC8 in mouse liver tissue lysate (lnae 1) and in HL-60 cell lysate (lane 2) .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the SIGLEC8 polyclonal antibody (Cat # PAB3640) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma. -
Gene Info — SIGLEC8
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Disease
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Publication Reference
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Molecular characterization of a Siglec8 variant containing cytoplasmic tyrosine-based motifs, and mapping of the Siglec8 gene.
Foussias G, Yousef GM, Diamandis EP.
Biochemical and Biophysical Research Communications 2000 Nov; 278(3):775.
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Identification of SAF-2, a novel siglec expressed on eosinophils, mast cells, and basophils.
Kikly KK, Bochner BS, Freeman SD, Tan KB, Gallagher KT, D'alessio KJ, Holmes SD, Abrahamson JA, Erickson-Miller CL, Murdock PR, Tachimoto H, Schleimer RP, White JR.
The Journal of Allergy and Clinical Immunology 2000 Jun; 105(6 Pt 1):1093.
Application:AP, Recombinant protein.
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Siglec-8. A novel eosinophil-specific member of the immunoglobulin superfamily.
Floyd H, Ni J, Cornish AL, Zeng Z, Liu D, Carter KC, Steel J, Crocker PR.
The Journal of Biological Chemistry 2000 Jan; 275(2):861.
Application:Flow Cyt, Human, Lymphocytes, Monocytes, Granulocytes.
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Molecular characterization of a Siglec8 variant containing cytoplasmic tyrosine-based motifs, and mapping of the Siglec8 gene.
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