PTPRC polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
The PTPRC polyclonal antibody (Cat # PAB3629) is used in Western blot to detect PTPRC in Jurkat cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human tonsil reacted with PTPRC polyclonal antibody (Cat # PAB3629), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow Cytometry
Flow cytometric analysis of Jurkat cells using PTPRC polyclonal antibody (Cat # PAB3629) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PTPRC.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human PTPRC.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Flow Cytometry (1:10-50)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:10-50)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
The PTPRC polyclonal antibody (Cat # PAB3629) is used in Western blot to detect PTPRC in Jurkat cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human tonsil reacted with PTPRC polyclonal antibody (Cat # PAB3629), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Flow Cytometry
Flow cytometric analysis of Jurkat cells using PTPRC polyclonal antibody (Cat # PAB3629) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. -
Gene Info — PTPRC
Entrez GeneID
5788Protein Accession#
P08575Gene Name
PTPRC
Gene Alias
B220, CD45, CD45R, GP180, LCA, LY5, T200
Gene Description
protein tyrosine phosphatase, receptor type, C
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq
Other Designations
CD45 antigen|T200 glycoprotein|T200 leukocyte common antigen|glycoprotein|leukocyte-common antigen|protein tyrosine phosphatase, receptor type, c polypeptide
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Interactome
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Pathway
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Publication Reference
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Potential Role of Caveolin-1 in Regulating the Function of Endothelial Progenitor Cells from Experimental MODS Model.
Luo T, Shu J, Lu Z, Han T, Fang G, Xue X.
Mediators of Inflammation 2019 Apr; 2019:8297391.
Application：Flow Cyt, Pig, Pig endothelial progenitor cells.
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A high-frequency polymorphism in exon 6 of the CD45 tyrosine phosphatase gene (PTPRC) resulting in altered isoform expression.
Stanton T, Boxall S, Hirai K, Dawes R, Tonks S, Yasui T, Kanaoka Y, Yuldasheva N, Ishiko O, Bodmer W, Beverley PC, Tchilian EZ.
PNAS 2003 Apr; 100(10):5997.
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Negative regulation of T cell activation by placental protein 14 is mediated by the tyrosine phosphatase receptor CD45.
Rachmilewitz J, Borovsky Z, Riely GJ, Miller R, Tykocinski ML.
The Journal of Biological Chemistry 2003 Apr; 278(16):14059.
Application：Flow Cyt, IF, Human, Jurkat, J45, CD4+ T cells.
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77 C/G mutation in the tyrosine phosphatase CD45 gene and autoimmune hepatitis: evidence for a genetic link.
Vogel A, Strassburg CP, Manns MP.
Genes and Immunity 2003 Jan; 4(1):79.
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Potential Role of Caveolin-1 in Regulating the Function of Endothelial Progenitor Cells from Experimental MODS Model.
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