HPCA polyclonal antibody
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More Files
- More Functions
Western Blot
The HPCA polyclonal antibody (Cat # PAB3607) is used in Western blot to detect HPCA in Y79 cell lysate (Lane 1) and mouse brain tissue lysate (Lane 2) .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with the HPCA polyclonal antibody (Cat # PAB3607) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human brain tissue reacted with HPCA polyclonal antibody (Cat # PAB3607) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of HPCA.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human HPCA.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
The HPCA polyclonal antibody (Cat # PAB3607) is used in Western blot to detect HPCA in Y79 cell lysate (Lane 1) and mouse brain tissue lysate (Lane 2) .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with the HPCA polyclonal antibody (Cat # PAB3607) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human brain tissue reacted with HPCA polyclonal antibody (Cat # PAB3607) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. -
Gene Info — HPCA
Entrez GeneID
3208Protein Accession#
P32076Gene Name
HPCA
Gene Alias
BDR2
Gene Description
hippocalcin
Omim ID
142622Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of neuron-specific calcium-binding proteins family found in the retina and brain. This protein is associated with the plasma membrane. It has similarities to proteins located in the photoreceptor cells that regulate photosignal transduction in a calcium-sensitive manner. This protein displays recoverin activity and a calcium-dependent inhibition of rhodopsin kinase. It is identical to the rat and mouse hippocalcin proteins and thought to play an important role in neurons of the central nervous system in a number of species. [provided by RefSeq
Other Designations
OTTHUMP00000004138|neuron specific calcium-binding protein hippocalcin
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Interactome
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Publication Reference
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Molecular cloning of human hippocalcin cDNA and chromosomal mapping of its gene.
Takamatsu K, Kobayashi M, Saitoh S, Fujishiro M, Noguchi T.
Biochemical and Biophysical Research Communications 1994 Apr; 200(1):606.
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Neurocalcin family: a novel calcium-binding protein abundant in bovine central nervous system.
Hidaka H, Okazaki K.
Neuroscience Research 1993 Feb; 16(2):73.
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Molecular cloning of hippocalcin, a novel calcium-binding protein of the recoverin family exclusively expressed in hippocampus.
Kobayashi M, Takamatsu K, Saitoh S, Miura M, Noguchi T.
Biochemical and Biophysical Research Communications 1992 Nov; 189(1):511.
Application：IHC, WB-Ti, Rat, Liver, Kidney, Brain, Retina.
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Molecular cloning of human hippocalcin cDNA and chromosomal mapping of its gene.
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