GJB2 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of GJB2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human GJB2.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Tissue lysate)
The GJB2 polyclonal antibody (Cat # PAB3582) is used in Western blot to detect GJB2 in mouse brain tissue lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with GJB2 polyclonal antibody (Cat # PAB3582) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — GJB2
Entrez GeneID
2706Protein Accession#
P29033Gene Name
GJB2
Gene Alias
CX26, DFNA3, DFNB1, HID, KID, NSRD1, PPK
Gene Description
gap junction protein, beta 2, 26kDa
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the gap junction protein family. The gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells. These structures were shown to consist of cell-to-cell channels that facilitate the transfer of ions and small molecules between cells. The gap junction proteins, also known as connexins, purified from fractions of enriched gap junctions from different tissues differ. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Mutations in this gene are responsible for as much as 50% of pre-lingual, recessive deafness. [provided by RefSeq
Other Designations
OTTHUMP00000018093|OTTHUMP00000018094|connexin 26|gap junction protein beta 2|gap junction protein, beta 2, 26kD (connexin 26)
-
Interactome
-
Disease
-
Publication Reference
-
Inhibition of Connexin 26 by the AMP-Activated Protein Kinase.
Alesutan I, Sopjani M, Munoz C, Fraser S, Kemp BE, Foller M, Lang F.
The Journal of Membrane Biology 2011 Apr; 240(3):151.
Application:IHC, Frog, Oocyte.
-
Novel mutations in GJB2 encoding connexin-26 in Japanese patients with keratitis-ichthyosis-deafness syndrome.
Yotsumoto S, Hashiguchi T, Chen X, Ohtake N, Tomitaka A, Akamatsu H, Matsunaga K, Shiraishi S, Miura H, Adachi J, Kanzaki T.
The British Journal of Dermatology 2003 Apr; 148(4):649.
-
GJB2 deafness gene shows a specific spectrum of mutations in Japan, including a frequent founder mutation.
Ohtsuka A, Yuge I, Kimura S, Namba A, Abe S, Van Laer L, Van Camp G, Usami S.
Human Genetics 2003 Apr; 112(4):329.
-
The novel R75Q mutation in the GJB2 gene causes autosomal dominant hearing loss and palmoplantar keratoderma in a Turkish family.
Uyguner O, Tukel T, Baykal C, Eris H, Emiroglu M, Hafiz G, Ghanbari A, Baserer N, Yuksel-Apak M, Wollnik B.
Clinical Genetics 2002 Oct; 62(4):306.
-
Inhibition of Connexin 26 by the AMP-Activated Protein Kinase.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com