GJA1 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of GJA1 polyclonal antibody (Cat # PAB3581) in HL-60 cell lysate. GJA1 (Arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with GJA1 polyclonal antibody (Cat # PAB3581) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of GJA1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human GJA1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of GJA1 polyclonal antibody (Cat # PAB3581) in HL-60 cell lysate. GJA1 (Arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with GJA1 polyclonal antibody (Cat # PAB3581) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma. -
Gene Info — GJA1
Entrez GeneID
2697Protein Accession#
P17302Gene Name
GJA1
Gene Alias
CX43, DFNB38, GJAL, ODDD
Gene Description
gap junction protein, alpha 1, 43kDa
Gene Ontology
HyperlinkGene Summary
This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia and heart malformations. [provided by RefSeq
Other Designations
OTTHUMP00000017111|connexin 43|gap junction 43 kDa heart protein|gap junction protein, alpha-like
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Interactome
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Pathway
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Disease
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Publication Reference
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Gating and regulation of connexin 43 (Cx43) hemichannels.
Contreras JE, Saez JC, Bukauskas FF, Bennett MV.
PNAS 2003 Sep; 100(20):11388.
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Endothelin-1 decreases gap junctional intercellular communication by inducing phosphorylation of connexin 43 in human ovarian carcinoma cells.
Spinella F, Rosano L, Di Castro V, Nicotra MR, Natali PG, Bagnato A.
The Journal of Biological Chemistry 2003 Oct; 278(42):41294.
Application：IF, IP, WB, Human, HEY, OVCA 433 cells.
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Lysosomal and proteasomal degradation play distinct roles in the life cycle of Cx43 in gap junctional intercellular communication-deficient and -competent breast tumor cells.
Qin H, Shao Q, Igdoura SA, Alaoui-Jamali MA, Laird DW.
The Journal of Biological Chemistry 2003 Aug; 278(32):30005.
Application：ICC, IEM, IF, WB, Human, BICR-M1Rk, MDA-MB-231 cells.
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Gating and regulation of connexin 43 (Cx43) hemichannels.
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