Tlr2 polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Tlr2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of mouse Tlr2.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Tlr2 polyclonal antibody (Cat # PAB3544) in CEM cell line lysate. TLR2-V735 (arrow) was detected using the purified polyclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with Tlr2 polyclonal antibody (Cat # PAB3544) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — Tlr2
Entrez GeneID
24088Protein Accession#
NP_036035;Q9QUN7Gene Name
Tlr2
Gene Alias
Ly105
Gene Description
toll-like receptor 2
Gene Ontology
HyperlinkOther Designations
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Interactome
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Publication Reference
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Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs.
Okazaki Y, Furuno M, Kasukawa T, Adachi J, Bono H, Kondo S, Nikaido I, Osato N, Saito R, Suzuki H, Yamanaka I, Kiyosawa H, Yagi K, Tomaru Y, Hasegawa Y, Nogami A, Schonbach C, Gojobori T, Baldarelli R, Hill DP, Bult C, Hume DA, Quackenbush J, Schriml LM, Kanapin A, Matsuda H, Batalov S, Beisel KW, Blake JA, Bradt D, Brusic V, Chothia C, Corbani LE, Cousins S, Dalla E, Dragani TA, Fletcher CF, Forrest A, Frazer KS, Gaasterland T, Gariboldi M, Gissi C, Godzik A, Gough J, Grimmond S, Gustincich S,.
Nature 2002 Dec; 75(3):481.
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The lipopolysaccharide-activated toll-like receptor (TLR)-4 induces synthesis of the closely related receptor TLR-2 in adipocytes.
Lin Y, Lee H, Berg AH, Lisanti MP, Shapiro L, Scherer PE.
The Journal of Biological Chemistry 2000 Aug; 275(32):24255.
Application:IP, WB-Ce, Human, Mouse, 293T, 3T3-L1 cells.
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Gene expressions of lipopolysaccharide receptors, toll-like receptors 2 and 4, are differently regulated in mouse T lymphocytes.
Matsuguchi T, Takagi K, Musikacharoen T, Yoshikai Y.
Blood 2000 Feb; 95(4):1378.
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Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs.
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