CDC2 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CDC2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human CDC2.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
Flow Cytometry (1:10-50)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of CDC2 polyclonal antibody (Cat # PAB3536) in 293 cell line lysates (35 ug/lane). CDC2 (arrow) was detected using the purified polyclonal antibody (1:1000 dilution).Western Blot (Transfected lysate)
Western blot analysis of CDC2 (arrow) using rabbit CDC2 polyclonal antibody (Cat # PAB3536). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDC2 gene (Lane 2) (Origene Technologies).Flow Cytometry
Flow cytometric analysis of 293 cells using CDC2 polyclonal antibody (Cat # PAB3536)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. -
Gene Info — CDC2
Entrez GeneID
983Protein Accession#
NP_001777;P06493Gene Name
CDC2
Gene Alias
CDC28A, CDK1, DKFZp686L20222, MGC111195
Gene Description
cell division cycle 2, G1 to S and G2 to M
Omim ID
116940Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000019660|cell cycle controller CDC2|cell division control protein 2 homolog|cell division cycle 2 protein|cyclin-dependent kinase 1|p34 protein kinase
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Pathway
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Publication Reference
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Control of mitotic exit by PP2A regulation of Cdc25C and Cdk1.
Forester CM, Maddox J, Louis JV, Goris J, Virshup DM.
PNAS 2007 Dec; 104(50):19867.
Application:WB-Tr, Human, HEK 293 cells.
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Mitosis persists in the absence of Cdk1 activity when proteolysis or protein phosphatase activity is suppressed.
Skoufias DA, Indorato RL, Lacroix F, Panopoulos A, Margolis RL.
The Journal of Cell Biology 2007 Nov; 179(4):671.
Application:IP-WB, Human, HeLa cells.
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Differential repression of c-myc and cdc2 gene expression by ERF and PE-1/METS.
Hester KD, Verhelle D, Escoubet-Lozach L, Luna R, Rose DW, Glass CK.
Cell Cycle 2007 Jul; 6(13):1594.
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Control of mitotic exit by PP2A regulation of Cdc25C and Cdk1.
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