Western blot analysis of CD4 polyclonal antibody (Cat # PAB3534) in HeLa cell line lysates (35 ug/lane). CD4 (arrow) was detected using the purified polyclonal antibody (1:1000 dilution).
Western Blot (Transfected lysate)
(RIGHT) Western blot analysis of CD4 (arrow) using rabbit CD4 polyclonal antibody (Cat # PAB3534). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD4 gene (Lane 2) (Origene Technologies).
Flow cytometric analysis of CEM cells using CD4 polyclonal antibody (Cat # PAB3534)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.