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Last updated: 2014/11/23
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CD4 polyclonal antibody

  • Catalog # : PAB3534
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of CD4.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human CD4.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Ammonium sulfate precipitation
  • Recommend Usage:
  • ELISA (1:1000)
    Western Blot (1:50-100)
    Immunohistochemistry (1:10-50)
    Flow cytometry (1:10-50)
    Immunofluorescence (1:10-50)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of CD4 polyclonal antibody (Cat # PAB3534) in HeLa cell line lysates (35 ug/lane). CD4 (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • (RIGHT) Western blot analysis of CD4 (arrow) using rabbit CD4 polyclonal antibody (Cat # PAB3534). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD4 gene (Lane 2) (Origene Technologies).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human lung carcinomareacted with CD4 polyclonal antibody (Cat # PAB3534), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
    This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • Immunofluorescence
  • ELISA
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of CEM cells using CD4 polyclonal antibody (Cat # PAB3534)(bottom histogram) compared to a negative control cell (top histogram).
    FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunofluorescence
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 920
  • Protein Accession#:
  • NP_000607;P01730
  • Gene Name:
  • CD4
  • Gene Alias:
  • CD4mut
  • Gene Description:
  • CD4 molecule
  • Other Designations:
  • CD4 antigen,CD4 antigen (p55),CD4 receptor
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