GCK polyclonal antibody
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More Files
- More Functions
Western Blot
The GCK polyclonal antibody (Cat # PAB3531) is used in Western blot to detect GCK in HepG2 cell lysate (Lane 1) and mouse liver tissue lysate (Lane 2) .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with GCK polyclonal antibody (Cat # PAB3531) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of GCK.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human GCK.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunofluorescence (1:10-50)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
The GCK polyclonal antibody (Cat # PAB3531) is used in Western blot to detect GCK in HepG2 cell lysate (Lane 1) and mouse liver tissue lysate (Lane 2) .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with GCK polyclonal antibody (Cat # PAB3531) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.Immunofluorescence
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Gene Info — GCK
Entrez GeneID
2645Protein Accession#
P35557Gene Name
GCK
Gene Alias
GK, GLK, HHF3, HK4, HKIV, HXKP, MODY2
Gene Description
glucokinase (hexokinase 4)
Gene Ontology
HyperlinkGene Summary
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI). [provided by RefSeq
Other Designations
ATP:D-hexose 6-phosphotransferase|OTTHUMP00000024521|OTTHUMP00000159308|glucokinase|hexokinase D, pancreatic isozyme
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Interactome
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Pathway
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Disease
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Publication Reference
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Insights into the biochemical and genetic basis of glucokinase activation from naturally occurring hypoglycemia mutations.
Gloyn AL, Noordam K, Willemsen MA, Ellard S, Lam WW, Campbell IW, Midgley P, Shiota C, Buettger C, Magnuson MA, Matschinsky FM, Hattersley AT.
Diabetes 2003 Sep; 52(9):2433.
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Genetic epidemiology of MODY in the Czech republic: new mutations in the MODY genes HNF-4alpha, GCK and HNF-1alpha.
Pruhova S, Ek J, Lebl J, Sumnik Z, Saudek F, Andel M, Pedersen O, Hansen T.
Diabetologia 2003 Feb; 46(2):291.
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A functional link between glucokinase binding to insulin granules and conformational alterations in response to glucose and insulin.
Rizzo MA, Magnuson MA, Drain PF, Piston DW.
The Journal of Biological Chemistry 2002 Sep; 277(37):34168.
Application：IF, WB, Mouse, βTC3 cells.
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Insights into the biochemical and genetic basis of glucokinase activation from naturally occurring hypoglycemia mutations.
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