SARS polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of SARS polyclonal antibody (Cat # PAB3517) in NCI-H460 cell line lysates (35 ug/lane). SARS (arrow) was detected using the purified polyclonal antibody (1:1000 dilution).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human brainreacted with SARS polyclonal antibody (Cat # PAB3517), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of SARS.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human SARS.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:10-50)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of SARS polyclonal antibody (Cat # PAB3517) in NCI-H460 cell line lysates (35 ug/lane). SARS (arrow) was detected using the purified polyclonal antibody (1:1000 dilution).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human brainreacted with SARS polyclonal antibody (Cat # PAB3517), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. -
Gene Info — SARS
Entrez GeneID
6301Protein Accession#
NP_006504;P49591Gene Name
SARS
Gene Alias
FLJ36399, SERRS, SERS
Gene Description
seryl-tRNA synthetase
Omim ID
607529Gene Ontology
HyperlinkGene Summary
This gene belongs to the class II amino-acyl tRNA family. The encoded enzyme catalyzes the transfer of L-serine to tRNA (Ser) and is related to bacterial and yeast counterparts. [provided by RefSeq
Other Designations
OTTHUMP00000013432|serine tRNA ligase 1, cytoplasmic|serine-tRNA ligase
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Interactome
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Pathway
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Disease
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Publication Reference
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Dual mode recognition of two isoacceptor tRNAs by mammalian mitochondrial seryl-tRNA synthetase.
Shimada N, Suzuki T, Watanabe K.
The Journal of Biological Chemistry 2001 Dec; 276(50):46770.
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Novel coding-region polymorphisms in mitochondrial seryl-tRNA synthetase (SARSM) and mitoribosomal protein S12 (RPMS12) genes in DFNA4 autosomal dominant deafness families.
Shah ZH, Toompuu M, Hakkinen T, Rovio AT, van Ravenswaay C, De Leenheer EM, Smith RJ, Cremers FP, Cremers CW, Jacobs HT.
Human Mutation 2001 May; 17(5):433.
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Minimal tRNA(Ser) and tRNA(Sec) substrates for human seryl-tRNA synthetase: contribution of tRNA domains to serylation and tertiary structure.
Heckl M, Busch K, Gross HJ.
FEBS Letters 1998 May; 427(3):315.
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Dual mode recognition of two isoacceptor tRNAs by mammalian mitochondrial seryl-tRNA synthetase.
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