LIMK1 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
The LIMK1 polyclonal antibody (Cat # PAB3484) is used in Western blot to detect LIMK1 in A-375 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with LIMK1 polyclonal antibody (Cat # PAB3484) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of LIMK1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human LIMK1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
The LIMK1 polyclonal antibody (Cat # PAB3484) is used in Western blot to detect LIMK1 in A-375 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with LIMK1 polyclonal antibody (Cat # PAB3484) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — LIMK1
Entrez GeneID
3984Protein Accession#
P53667Gene Name
LIMK1
Gene Alias
LIMK
Gene Description
LIM domain kinase 1
Omim ID
601329Gene Ontology
HyperlinkGene Summary
There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is likely to be a component of an intracellular signaling pathway and may be involved in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. [provided by RefSeq
Other Designations
LIM motif-containing protein kinase|OTTHUMP00000025066
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
Rho-associated kinase ROCK activates LIM-kinase 1 by phosphorylation at threonine 508 within the activation loop.
Ohashi K, Nagata K, Maekawa M, Ishizaki T, Narumiya S, Mizuno K.
The Journal of Biological Chemistry 2000 Feb; 275(5):3577.
Application：WB-Tr, Monkey, COS-7 cells.
-
Signaling from Rho to the actin cytoskeleton through protein kinases ROCK and LIM-kinase.
Maekawa M, Ishizaki T, Boku S, Watanabe N, Fujita A, Iwamatsu A, Obinata T, Ohashi K, Mizuno K, Narumiya S.
Science 1999 Aug; 285(5429):895.
-
Structural features of LIM kinase that control effects on the actin cytoskeleton.
Edwards DC, Gill GN.
The Journal of Biological Chemistry 1999 Apr; 274(16):11352.
Application：IF, IP, KA, WB-Tr, Monkey, COS-7, HEK 293 cells.
-
Rho-associated kinase ROCK activates LIM-kinase 1 by phosphorylation at threonine 508 within the activation loop.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com