ARAF polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ARAF.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to internal region of human ARAF.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of MCF-7 cell lysate (35 ug/lane) with ARAF polyclonal antibody (Cat # PAB3478).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with ARAF polyclonal antibody (Cat # PAB3478) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma. -
Gene Info — ARAF
Entrez GeneID
369Protein Accession#
P10398Gene Name
ARAF
Gene Alias
A-RAF, ARAF1, PKS2, RAFA1
Gene Description
v-raf murine sarcoma 3611 viral oncogene homolog
Omim ID
311010Gene Ontology
HyperlinkOther Designations
OTTHUMP00000023212|Oncogene ARAF1|Ras-binding protein DA-Raf|v-raf murine sarcoma 3611 viral oncogene homolog 1
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Interactome
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Pathway
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Publication Reference
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Selective activation of MEK1 but not MEK2 by A-Raf from epidermal growth factor-stimulated Hela cells.
Wu X, Noh SJ, Zhou G, Dixon JE, Guan KL.
The Journal of Biological Chemistry 1996 Feb; 271(6):3265.
Application:IP-WB, Human, HeLa cells.
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The complete sequence and promoter activity of the human A-raf-1 gene (ARAF1).
Lee JE, Beck TW, Brennscheidt U, DeGennaro LJ, Rapp UR.
Genomics 1994 Mar; 20(1):43.
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Selective activation of MEK1 but not MEK2 by A-Raf from epidermal growth factor-stimulated Hela cells.
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