ACVRL1 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
Western blot analysis of ACVRL1 polyclonal antibody (Cat # PAB3475) in mouse heart tissue lysate.
Western Blot (Transfected lysate)
Western blot analysis of ACVRL1 (arrow) using rabbit ACVRL1 polyclonal antibody (Cat # PAB3475). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ACVRL1 gene (Lane 2) (Origene Technologies).
Western Blot (Transfected lysate)
Western blot analysis of ACVRL1 polyclonal antibody (Cat # PAB3475) in human chondrocytes (C-28/I2 cells), transfected with empty vector (lane 1, 3) or ACVRL1 (lane 2, 4). RIPA lysis buffer, 20 ug/lane of protein, primary antibody dilution 1 : 1000, blocking solution is 5% milk in TBST (lane 1 and 2), 5% BSA in TBST (lane 3 and 4).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with ACVRL1 polyclonal antibody (Cat # PAB3475) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
Flow Cytometry
Flow cytometric analysis of HepG2 cells using ACVRL1 polyclonal antibody (Cat # PAB3475)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ACVRL1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human ACVRL1.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
Flow Cytometry (1:10-50)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of ACVRL1 polyclonal antibody (Cat # PAB3475) in mouse heart tissue lysate.Western Blot (Transfected lysate)
Western blot analysis of ACVRL1 (arrow) using rabbit ACVRL1 polyclonal antibody (Cat # PAB3475). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ACVRL1 gene (Lane 2) (Origene Technologies).Western Blot (Transfected lysate)
Western blot analysis of ACVRL1 polyclonal antibody (Cat # PAB3475) in human chondrocytes (C-28/I2 cells), transfected with empty vector (lane 1, 3) or ACVRL1 (lane 2, 4). RIPA lysis buffer, 20 ug/lane of protein, primary antibody dilution 1 : 1000, blocking solution is 5% milk in TBST (lane 1 and 2), 5% BSA in TBST (lane 3 and 4).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with ACVRL1 polyclonal antibody (Cat # PAB3475) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.Flow Cytometry
Flow cytometric analysis of HepG2 cells using ACVRL1 polyclonal antibody (Cat # PAB3475)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. -
Gene Info — ACVRL1
Entrez GeneID
94Protein Accession#
P37023Gene Name
ACVRL1
Gene Alias
ACVRLK1, ALK-1, ALK1, HHT, HHT2, ORW2, SKR3, TSR-I
Gene Description
activin A receptor type II-like 1
Gene Ontology
HyperlinkGene Summary
This gene encodes a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. The encoded protein, sometimes termed ALK1, shares similar domain structures with other closely related ALK or activin receptor-like kinase proteins that form a subfamily of receptor serine/threonine kinases. Mutations in this gene are associated with hemorrhagic telangiectasia type 2, also known as Rendu-Osler-Weber syndrome 2. [provided by RefSeq
Other Designations
TGF-B superfamily receptor type I|activin A receptor, type II-like kinase 1|serine/threonine-protein kinase receptor R3
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Interactome
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Pathway
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Disease
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Publication Reference
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LC-MS/MS analysis of apical and basolateral plasma membranes of rat renal collecting duct cells.
Yu MJ, Pisitkun T, Wang G, Shen RF, Knepper MA.
Molecular & Cellular Proteomics 2006 Aug; 5(11):2131.
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LC-MS/MS analysis of apical and basolateral plasma membranes of rat renal collecting duct cells.
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