IRAK2 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of IRAK2 polyclonal antibody (Cat # PAB3471) in K-562 cell lysate. IRAK2 (Arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with IRAK2 polyclonal antibody (Cat # PAB3471) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of IRAK2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human IRAK2.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of IRAK2 polyclonal antibody (Cat # PAB3471) in K-562 cell lysate. IRAK2 (Arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with IRAK2 polyclonal antibody (Cat # PAB3471) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — IRAK2
Entrez GeneID
3656Protein Accession#
O43187Gene Name
IRAK2
Gene Alias
IRAK-2, MGC150550
Gene Description
interleukin-1 receptor-associated kinase 2
Omim ID
603304Gene Ontology
HyperlinkGene Summary
IRAK2 encodes the interleukin-1 receptor-associated kinase 2, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. IRAK2 is reported to participate in the IL1-induced upregulation of NF-kappaB. [provided by RefSeq
Other Designations
interleukin-1 receptor associated kinase-2
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Interactome
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Pathway
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Disease
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Publication Reference
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Identification and characterization of murine IRAK-M.
Rosati O, Martin MU.
Biochemical and Biophysical Research Communications 2002 May; 293(5):1472.
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Identification and characterization of murine IRAK-M.
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