NRAS polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of NRAS.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human NRAS.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
Flow Cytometry (1:10-50)
Immunofluorescence (1:10-50)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of NRAS polyclonal antibody (Cat # PAB3461) in CEM cell line lysates (35 ug/lane). NRAS (arrow) was detected using the purified polyclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of NRAS (arrow) using rabbit NRAS polyclonal antibody (Cat # PAB3461). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NRAS gene (Lane 2) (Origene Technologies).Immunofluorescence
Flow Cytometry
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Gene Info — NRAS
Entrez GeneID
4893Protein Accession#
NP_002515;P01111Gene Name
NRAS
Gene Alias
ALPS4, N-ras, NRAS1
Gene Description
neuroblastoma RAS viral (v-ras) oncogene homolog
Gene Ontology
HyperlinkGene Summary
This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Defects in this gene are a cause of juvenile myelomonocytic leukemia (JMML). [provided by RefSeq
Other Designations
N-ras protein part 4|OTTHUMP00000013879|v-ras neuroblastoma RAS viral oncogene homolog
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Interactome
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Pathway
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Disease
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Publication Reference
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Identification of a novel subgroup of melanomas with KIT/cyclin-dependent kinase-4 overexpression.
Smalley KS, Contractor R, Nguyen TK, Xiao M, Edwards R, Muthusamy V, King AJ, Flaherty KT, Bosenberg M, Herlyn M, Nathanson KL.
Cancer Research 2008 Jul; 68(14):5743.
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BRAF and NRAS mutations in melanoma: potential relationships to clinical response to HSP90 inhibitors.
Banerji U, Affolter A, Judson I, Marais R, Workman P.
Molecular Cancer Therapeutics 2008 Mar; 7(4):737.
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Identification of a novel subgroup of melanomas with KIT/cyclin-dependent kinase-4 overexpression.
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