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Last updated: 2014/8/25
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BTK polyclonal antibody

  • Catalog # : PAB3419
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of BTK.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to internal region of human BTK.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Recommend Usage:
  • ELISA (1:1000)
    Western Blot (1:100-500)
    Immunohistochemistry (1:50-100)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • The BTK polyclonal antibody (Cat # PAB3419) is used in Western blot to detect BTK in Ramos cell lysate.
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis of BTK polyclonal antibody (Cat # PAB3419) transiently transfected HEK293 cell line lysate (1 ug/lane). BTK polyclonal antibody (arrow) was detected using the purified polyclonal antibody (1 : 1500 dilution).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human cancer tissue reacted with BTK polyclonal antibody (Cat # PAB3419) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 695
  • Gene Name:
  • BTK
  • Gene Alias:
  • AGMX1,AT,ATK,BPK,IMD1,MGC126261,MGC126262,PSCTK1,XLA
  • Gene Description:
  • Bruton agammaglobulinemia tyrosine kinase
  • Gene Summary:
  • The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. [provided by RefSeq
  • Other Designations:
  • B-cell progenitor kinase,Bruton tyrosine kinase,OTTHUMP00000023676,OTTHUMP00000063593,agammaglobulinemia tyrosine kinase,dominant-negative kinase-deficient Brutons tyrosine kinase,tyrosine-protein kinase BTK
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