BUB1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of BUB1 polyclonal antibody (Cat # PAB3262) in T-47D cell line lysate. BUB1 (arrow) was detected using the purified polyclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with BUB1 polyclonal antibody (Cat # PAB3262) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of BUB1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human BUB1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of BUB1 polyclonal antibody (Cat # PAB3262) in T-47D cell line lysate. BUB1 (arrow) was detected using the purified polyclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with BUB1 polyclonal antibody (Cat # PAB3262) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma. -
Gene Info — BUB1
Entrez GeneID
699Protein Accession#
O43683Gene Name
BUB1
Gene Alias
BUB1A, BUB1L, hBUB1
Gene Description
budding uninhibited by benzimidazoles 1 homolog (yeast)
Omim ID
602452Gene Ontology
HyperlinkGene Summary
This gene encodes a kinase involved in spindle checkpoint function. The kinase functions in part by phosphorylating a member of the miotic checkpoint complex and activating the spindle checkpoint. Mutations in this gene have been associated with aneuploidy and several forms of cancer. [provided by RefSeq
Other Designations
BUB1 budding uninhibited by benzimidazoles 1 homolog|budding uninhibited by benzimidazoles 1|mitotic spindle checkpoint kinase|putative serine/threonine-protein kinase
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Interactome
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Pathway
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Disease
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Publication Reference
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The beta2-adaptin clathrin adaptor interacts with the mitotic checkpoint kinase BubR1.
Cayrol C, Cougoule C, Wright M.
Biochemical and Biophysical Research Communications 2002 Nov; 298(5):720.
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Genetic and epigenetic inactivation of mitotic checkpoint genes hBUB1 and hBUBR1 and their relationship to survival.
Shichiri M, Yoshinaga K, Hisatomi H, Sugihara K, Hirata Y.
Cancer Research 2002 Jan; 62(1):13.
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The beta2-adaptin clathrin adaptor interacts with the mitotic checkpoint kinase BubR1.
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