ACTB/ACTC1 polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ACTB/ACTC1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to internal region of human ACTB/ACTC1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of ACTB/ACTC1 polyclonal antibody (Cat # PAB3161) in HL-60 cell line lysates (35 ug/lane). ACTB/ACTC1 (arrow) was detected using the purified polyclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of ACTB/ACTC (arrow) using rabbit ACTB/ACTC1 polyclonal antibody (Cat # PAB3161). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ACTB/ACTC gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with ACTB/ACTC1 polyclonal antibody (Cat # PAB3161) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. -
Gene Info — ACTB
Entrez GeneID
60Protein Accession#
NP_001092 (Gene ID : 60);P60709 (Gene ID : 60);NP_005150 (Gene ID : 70);P68032 (Gene ID : 70)Gene Name
ACTB
Gene Alias
PS1TP5BP1
Gene Description
actin, beta
Omim ID
102630Gene Ontology
HyperlinkGene Summary
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq
Other Designations
PS1TP5-binding protein 1|actin, cytoplasmic 1|beta actin|beta cytoskeletal actin
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Gene Info — ACTC1
Entrez GeneID
70Protein Accession#
NP_001092 (Gene ID : 60);P60709 (Gene ID : 60);NP_005150 (Gene ID : 70);P68032 (Gene ID : 70)Gene Name
ACTC1
Gene Alias
ACTC, CMD1R, CMH11
Gene Description
actin, alpha, cardiac muscle 1
Gene Ontology
HyperlinkGene Summary
Actins are highly conserved proteins that are involved in various types of cell motility. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to four others. The protein encoded by this gene belongs to the actin family which is comprised of three main groups of actin isoforms, alpha, beta, and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Defects in this gene have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC). [provided by RefSeq
Other Designations
cardiac muscle alpha actin 1
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Interactome
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Pathway
- Adherens junction
- Arrhythmogenic right ventricular cardiomyopathy (ARVC)
- Cardiac muscle contraction
- Focal adhesion
- Hypertrophic cardiomyopathy (HCM)
- Hypertrophic cardiomyopathy (HCM)
- Leukocyte transendothelial migration
- Pathogenic Escherichia coli infection - EHEC
- Regulation of actin cytoskeleton
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Disease
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Publication Reference
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Domain-specific chaperone-induced expansion is required for beta-actin folding: a comparison of beta-actin conformations upon interactions with GroEL and tail-less complex polypeptide 1 ring complex (TRiC).
Villebeck L, Moparthi SB, Lindgren M, Hammarstrom P, Jonsson BH.
Biochemistry 2007 Nov; 46(44):12639.
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An active Src kinase-beta-actin association is linked to actin dynamics at the periphery of colon cancer cells.
Avizienyte E, Keppler M, Sandilands E, Brunton VG, Winder SJ, Ng T, Frame MC.
Experimental Cell Research 2007 Sep; 313(15):3175.
Application:IP, WB, Human, KM12C cells.
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Variable presentation of nemaline myopathy: novel mutation of alpha actin gene.
Bouldin AA, Parisi MA, Laing N, Patterson K, Gospe SM Jr.
Muscle & Nerve 2007 Feb; 35(2):254.
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Domain-specific chaperone-induced expansion is required for beta-actin folding: a comparison of beta-actin conformations upon interactions with GroEL and tail-less complex polypeptide 1 ring complex (TRiC).
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