CDCA8 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of CDCA8 polyclonal antibody (Cat # PAB3096) in HL-60 cell line lysates (35 ug/lane). CDCA8 (arrow) was detected using the purified polyclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CDCA8 polyclonal antibody (Cat # PAB3096) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow Cytometry
Flow cytometric analysis of HL-60 cells using CDCA8 polyclonal antibody (Cat # PAB3096)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CDCA8.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to internal region of human CDCA8.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:10-50)
Flow cytometry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of CDCA8 polyclonal antibody (Cat # PAB3096) in HL-60 cell line lysates (35 ug/lane). CDCA8 (arrow) was detected using the purified polyclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CDCA8 polyclonal antibody (Cat # PAB3096) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Flow Cytometry
Flow cytometric analysis of HL-60 cells using CDCA8 polyclonal antibody (Cat # PAB3096)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. -
Gene Info — CDCA8
Entrez GeneID
55143Protein Accession#
NP_060571;Q53HL2Gene Name
CDCA8
Gene Alias
BOR, BOREALIN, FLJ10468, FLJ12042
Gene Description
cell division cycle associated 8
Omim ID
609977Gene Ontology
HyperlinkGene Summary
CDCA8 is a component of a chromosomal passenger complex required for stability of the bipolar mitotic spindle (Gassmann et al., 2004 [PubMed 15249581]).[supplied by OMIM
Other Designations
Dasra B|OTTHUMP00000004514|OTTHUMP00000004515
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Interactome
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Publication Reference
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Extensive Crosstalk Between O-GlcNAcylation and Phosphorylation Regulates Cytokinesis.
Wang Z, Udeshi ND, Slawson C, Compton PD, Sakabe K, Cheung WD, Shabanowitz J, Hunt DF, Hart GW.
Science Signaling 2010 Jan; 3(104):ra2.
Application：WB-Ce, Human, HeLa cells.
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A survivin-ran complex regulates spindle formation in tumor cells.
Xia F, Canovas PM, Guadagno TM, Altieri DC.
Molecular and Cellular Biology 2008 Jun; 28(17):5299.
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Extensive Crosstalk Between O-GlcNAcylation and Phosphorylation Regulates Cytokinesis.
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