Western blot analysis of ALDH2 (arrow) using rabbit ALDH2 polyclonal antibody (Cat # PAB3078). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ALDH2 gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with ALDH2 polyclonal antibody (Cat # PAB3078) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. [provided by RefSeq