The FGFR2 polyclonal antibody (Cat # PAB3041) is used in Western blot to detect FGFR2 in Jurkat cell lysate.
Western Blot (Transfected lysate)
Western blot analysis of FGFR2 polyclonal antibody (Cat # PAB3041) in 293 cell line lysates transiently transfected with the FGFR2 gene (2 ug/lane). hFGFR2-R22 (arrow) was detected using the purified polyclonal antibody (1 : 30 dilution).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with FGFR2 polyclonal antibody (Cat # PAB3041) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
FGFR2/isolectinB4 (A) and FGFR1/isolectinB4 (B) staining of apparent mesenchymal cells and the subpopulation of endothelial cells. Virtually all other dispersed apparent mesenchymal cells express FGFR1 and FGFR2 (merged image in C). F: FGFR2 (D) and FGFR1 (E) staining in clustered cells of epithelial origin (inferred by morphology here) demonstrating that epithelial cells express both FGFR1 and FGFR2 (merged image with DAPI staining in F).
The protein encoded by this gene is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member is a high-affinity receptor for acidic, basic and/or keratinocyte growth factor, depending on the isoform. Mutations in this gene are associated with Crouzon syndrome, Pfeiffer syndrome, Craniosynostosis, Apert syndrome, Jackson-Weiss syndrome, Beare-Stevenson cutis gyrata syndrome, Saethre-Chotzen syndrome, and syndromic craniosynostosis. Multiple alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq