EPHB3 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of EPHB3 polyclonal antibody (Cat # PAB3021) in Jurkat cell lysate. EPHB3 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with EPHB3 polyclonal antibody (Cat # PAB3021) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of EPHB3.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human EPHB3.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of EPHB3 polyclonal antibody (Cat # PAB3021) in Jurkat cell lysate. EPHB3 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with EPHB3 polyclonal antibody (Cat # PAB3021) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma. -
Gene Info — EPHB3
Entrez GeneID
2049Protein Accession#
P54753Gene Name
EPHB3
Gene Alias
ETK2, HEK2, TYRO6
Gene Description
EPH receptor B3
Omim ID
601839Gene Ontology
HyperlinkGene Summary
Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members. [provided by RefSeq
Other Designations
EPH-like tyrosine kinase-2|ephrin receptor EphB3|human embryo kinase 2
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Interactome
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Pathway
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Disease
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Publication Reference
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PCR mediated detection of a new human receptor-tyrosine-kinase, HEK 2.
Bohme B, Holtrich U, Wolf G, Luzius H, Grzeschik KH, Strebhardt K, Rubsamen-Waigmann H.
Oncogene 1993 Oct; 8(10):2857.
Application：WB-Ce, Human, A431 cells.
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PCR mediated detection of a new human receptor-tyrosine-kinase, HEK 2.
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