EPHA5 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of lysate from U-251 MG cell line, using EPHA5 polyclonal antibody was diluted at 1:1000. A goat anti-mouse IgG H&L (HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 35 ug.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human brain tissue reacted with EPHA5 polyclonal antibody (Cat # PAB3009) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of EPHA5.
Immunogen
Recombinant His fusion protein corresponding to human EPHA5.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA
Immunohistochemistry (1:10~50)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of lysate from U-251 MG cell line, using EPHA5 polyclonal antibody was diluted at 1:1000. A goat anti-mouse IgG H&L (HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 35 ug.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human brain tissue reacted with EPHA5 polyclonal antibody (Cat # PAB3009) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Enzyme-linked Immunoabsorbent Assay
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Gene Info — EPHA5
Entrez GeneID
2044Protein Accession#
P54756Gene Name
EPHA5
Gene Alias
CEK7, EHK1, HEK7, TYRO4
Gene Description
EPH receptor A5
Omim ID
600004Gene Ontology
HyperlinkGene Summary
This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Two transcript variants encoding different isoforms have been found for this gene.
Other Designations
Eph homology kinase-1|ephrin receptor EphA5|ephrin type-A receptor 5|receptor protein-tyrosine kinase HEK7|tyrosine-protein kinase receptor EHK-1
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Interactome
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Pathway
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Disease
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Publication Reference
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Expression profiling of the ovarian surface kinome reveals candidate genes for early neoplastic changes.
Pejovic T, Pande NT, Mori M, Mhawech-Fauceglia P, Harrington C, Mongoue-Tchokote S, Dim D, Andrews C, Beck A, Tarumi Y, Djilas J, Cappuccini F, Caballero O, Huang J, Levy S, Tsiamouri A, Cain J, Bagby GC, Strausberg RL, Simpson AJ, Odunsi KO.
Translational Oncology 2009 Dec; 2(4):341.
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cDNA cloning and tissue distribution of five human EPH-like receptor protein-tyrosine kinases.
Fox GM, Holst PL, Chute HT, Lindberg RA, Janssen AM, Basu R, Welcher AA.
Oncogene 1995 Mar; 10(5):897.
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Expression profiling of the ovarian surface kinome reveals candidate genes for early neoplastic changes.
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