Western blot analysis of EPHA4 polyclonal antibody (Cat # PAB3007) in HeLa cell lysate. EPHA4 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Western Blot (Transfected lysate)
Western blot analysis of EPHA4 (arrow) using EPHA4 polyclonal antibody (Cat # PAB3007). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EPHA4 gene (Lane 2).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with EPHA4 polyclonal antibody (Cat # PAB3007) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
Methanol/Acetone fixed human stem cell is used in IF to detect EPHA4 (blue) and endothelial Lectin (red) . Data kindly provided by Dr. Weis from Cheresh Lab, UCSD.
Flow cytometric analysis of CEM cells using EPHA4 polyclonal antibody (Cat # PAB3007)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. [provided by RefSeq