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EPHA4 polyclonal antibody

  • Catalog # : PAB3007
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of EPHA4.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human EPHA4.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Recommend Usage:
  • ELISA (1:1000)
    Western Blot (1:100-500)
    Immunohistochemistry (1:50-100)
    Immunofluorescence (1:100)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of EPHA4 polyclonal antibody (Cat # PAB3007) in HeLa cell lysate. EPHA4 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis of EPHA4 (arrow) using EPHA4 polyclonal antibody (Cat # PAB3007).
    293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EPHA4 gene (Lane 2).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human cancer tissue reacted with EPHA4 polyclonal antibody (Cat # PAB3007) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
  • Immunofluorescence
  • Immunofluorescence
  • Methanol/Acetone fixed human stem cell is used in IF to detect EPHA4 (blue) and endothelial Lectin (red) . Data kindly provided by Dr. Weis from Cheresh Lab, UCSD.
  • ELISA
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of CEM cells using EPHA4 polyclonal antibody (Cat # PAB3007)(bottom histogram) compared to a negative control cell (top histogram).
    FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 2043
  • Gene Name:
  • EPHA4
  • Gene Alias:
  • HEK8,SEK,TYRO1
  • Gene Description:
  • EPH receptor A4
  • Gene Summary:
  • This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000164185,TYRO1 protein tyrosine kinase,ephrin receptor EphA4,ephrin type-A receptor 4,receptor protein-tyrosine kinase HEK8,tyrosine-protein kinase receptor SEK
  • Gene Pathway
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